NF-κB and Nrf2 signaling pathways contribute to gamma-irradiated fibroin protein-mediated inhibition of TNF-α-induced oxidative stress and inflammation in skin keratinocytes
Mi-Jin Kwon1, Ju-Woon Lee2,5, Young Ho Cho3, Kwan-Soo Kim4, Sang-Chul Kim5, Hao Chen6, Chengbi Cui7.
1R&D Center, PSA CO. LTD, Pusan, Korea; 2Headquarter, PSA CO. LTD, Pusan, Korea; 3Department of Pharmacy and Biotechnology, Konyang University, Daejeon, Korea; 4Headquarter, Greenpia Technology Inc, Yeoju, Korea; 5R&D Center, Greenpia Technology Inc, Yeoju, Korea; 6Headquarter, Sichuan Institute of Atomic Energy, Chengdu, P.R. China; 7Department of Food Science and Nutrition, Yanbian University, Yanji, P.R. China
OBJECTIVES
Silk fibroin protein (FP) has been used for edible and cosmetic products (Asakura et al., 2004). Gamma irradiation improves physiological activity through the modification of the structure of FP (Byun et al., 2010). In this study, we examined whether gamma-irradiated FP (gFP) has a protective effect on TNF-α-induced cellular stress.
EXPERIMENTAL RESULTS
We investigated that FP changed the structure by gamma irradiation through electrophoresis, UV spectrum, and FT-IR. We further investigated the cytoprotective potential of 20 kGy-irradiated FP (gFP20) in HaCaT keratinocytes exposed to extracellular stress.
gFP20 effectively decreased TNFα-induced matrix metalloproteinase (MMP)-1 overexpression. In HaCat cells, gFP20 inhibited the espression of NFAT5/NF-κB by TNF-α, whiah is decreased level of inflammatory mediator and pro-inflammatory cytokines (Rallis et al., 2017). Furthermore, gFP20 protected cells from TNF-α-induced oxidative stress by attenuating ROS overexpression and increasing the expression of the HO-1 antioxidant enzyme as the result of the stimulation of translocation into the nucleus of Nrf2 (Kobayashi et al., 2016).
FP with low-dose irradiation may be a potential prevention agent with great efficacy for treating oxidative stress and inflammation in skin disease. Taken together, our findings indicate that gFP20 could be considered as a superior functional material of skin-care materials that inhibits MMP-1 and increases collagen.
REFERENCES
Asakura, T., et al., 2004. Mag. Reson. Chem. 42, 258-266.
Byun, E.B., et al., 2010. Chem. Biol. Interact. 186, 90-95.
Kobayashi, E.H., et al., 2016. Nat. Commun. 7, 11624.
Rallis, M., et al., 2017. Free Rad. Biol. Med. 108.
