Hyaluronic acid is a water-soluble polysaccharide with characteristics such as high viscosity, moisturizing ability, biocompatibility. It can be also effective in preventing skin wrinkles. However, its high molecular weight more than 1,000kDa makes it difficult to penetrate epidermis to deliver its functional effects. To overcome this disadvantage, hyaluronic acid was irradiated using 10MeV E-Beam to decrease its molecular weight to less than 100kDa and its effects on transdermal absorption was examined. The molecular weight of hyaluronic acid was measured using gel permeation chromatography. The FTIR spectrum showed no substantial changes of the spectral pattern between before and after electron beam irradiation. To confirm the transdermal absorption ability depending on the molecular weight and content of hyaluronic acid, 3D skin absorption test and anti-aging human efficacy evaluation were performed. In addition, biostability of the materials was proved through the human skin irritation evaluation. In conclusion, low molecular hyaluronic acid is considered to be promising for the functional material of dermacomestic patches due to the biostability and high transdermal transmittance.