Posters - Networking - Exhibition

Wednesday November 09, 2022 from 13:30 to 17:00


POS-13 Comparison of the microbicidal effectiveness of 150 kV x-rays and cobalt-60 gamma rays

Jakob G. Hjørringgaard, Denmark

Development Engineer
Health Technology
Technical University of Denmark


Comparison of the microbicidal effectiveness of 150 kV x-rays and cobalt-60 gamma rays

Jakob Hjørringgaard1, Arne Miller1, Claus E. Andersen1, Dominique Cloetta2, Willi Wandfluh2, Alan Tallentire3.

1Department of Health Technology, Technical University of Denmark, Roskilde, Denmark; 2ebeam Technologies, COMET AG, Flamatt, Switzerland; 3Independent, Wilmslow, United Kingdom


In two previous papers it has been shown that the dose response of Bacillus pumilus spores, irradiated under specified standardized conditions, is independent of beam quality for  a wide range of beam qualities (Tallentire et al., 2010; Tallentire and Miller, 2015). To expand the range of beam qualities covered by the conclusions of the previous papers we investigate the response of B. pumilus spores subjected to irradiation in a 150 kV x-ray beam.


Microbiological test pieces comprised of test filters with a known number of hydrated B. pumilus spores located on the surface of a GS grade cellulose acetate membrane filter placed in a Petri dish was used.

X-irradiation of test pieces was done using an XBA-200/270H x-ray tube. Gamma irradiation of test pieces was performed in a Nordion GC-220 Gammacell. The dose to test filters was measured during irradiation using both alanine film and pellet dosimeters. The alanine dosimeters are calibrated in cobalt-60 with traceability to NPL.

The survival curves obtained from irradiation in the two radiation fields are shown in Figure 1. It is evident that a common response function can describe the survivability of B. pumilus spores in these beam qualities.


The radiation response of water-hydrated B. pumilus spores to a 150 kV x-ray beam was found to be identical to the response to cobalt-60 gamma rays within experimental uncertainties.


Tallentire A, Miller A. Microbicidal effectiveness of x-rays used for sterilization purposes. Radiation Physics and Chemistry 2015;107:128-30.

Tallentire A, Miller A, Helt-Hansen J. A comparison of the microbicidal effectiveness of gamma rays and high and low energy electron radiation. Radiation Physics and Chemistry 2010;79(6):701-4.

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