Corchorus olitorius L. (Molokhia leaf) extract irradiated with gamma rays attenuate muscle atrophy via regulating protein turnover and mitochondrial biogenesis in C2C12 cells
Mi-Jin Kwon1, Ju-Woon Lee2,5, Young Ho Cho3, Kwan-Soo Kim4, Sang-Chul Kim5, Hao Chen6, Chengbi Cui7.
1R&D Center, PSA CO. LTD, Pusan, Korea; 2Headquarter, PSA CO. LTD, Pusan, Korea; 3Department of Pharmacy and Biotechnology, Konyang University, Daejeon, Korea; 4Headquarter, Greenpia Technology Inc, Yeoju, Korea; 5R&D Center, Greenpia Technology Inc, Yeoju, Korea; 6Headquarter, Sichuan Institute of Atomic Energy, Chengdu, P.R. China; 7Department of Food Science and Nutrition, Yanbian University, Yanji, P.R. China
OBJECTIVES
Sarcopenia is a disease of progressive loss of muscle mass due to imbalance of protein synthesis and proteolysis, and tends to emerge with ageing (Bonaldo et al., 2013). It is important to search for effective herbal medicines that can modulate muscle mass.(Yoshioka et al., 2019). Corchorus Olitorius L. (Molokhia leaf) is an Egyptian wide spread edible vegetable having high physiological activity. In this study, we investigated the inhibition effects of gamma-irradiated leaf extract (g-MOL) on dexamethasone-induced muscle atrophy in differentiation of C2C12 cells.
EXPERIMENTAL RESULTS
MOL (100 mg/mL) was irradiated in a Co-60 irradiator. g-MOL solution was used in each experiment by treating C2C12 cells with dexamethasone (DEX) to induce muscle atrophy.
We tested if g-MOL exerted an anti-atrophic effect on cultured C2C12 myotubes with DEX. Treatment of cultured myotubes with DEX reduced myotube size and increased proteosome activity, which were attenuated by g-MOL. Also, g-MOL effectively prevented dephosphorylation of forkhead box O 3α and upregulation of muscle-specific ubiquitin ligases in DEX-treated myotubes.
The protective effect of g-MOL on DEX-mediated myotube atrophy was regulated by insulin-like growth factor-1-dependent signaling. As well, g-MOL-stimulated mitochondrial DNA content via SIRT1/PGC-1α signaling. These findings suggest g-MOL could be used as a valuable natural material that inhibits skeletal muscle atrophy via regulating protein turnover and mitochondrial biogenesis (Martinez et al., 2018).
REFERENCES
Bonaldo P, et al., 2013. Dis. Model Mech. 6,25–39.
Martinez, M. I. Y., et al., 2018. Int. J. Endocrinol. 2018, 7816806.
Yoshioka, Y., et a., 2019. Arch. Biochem. Biophys. 664, 157–166 .